Identification of a lambda toxin-negative Clostridium perfringens strain that processes and activates epsilon prototoxin intracellularly
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چکیده
منابع مشابه
In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B
Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective proteinexpression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genesthat was used for cloning in E.coli. The etx and cpb genes were...
متن کاملTryptophan content of Clostridium perfringens epsilon toxin.
The tryptophan content of Clostridium perfringens epsilon toxin was investigated. When the tryptophan content was determined by amino acid analysis after the hydrolysis of epsilon prototoxin with methanesulfonic acid containing 3-(2-aminoethyl)indole and by the spectrophotometric method with N-bromosuccinimide, the number of tryptophan residues was calculated at 1/mol of the protein. Cleavage o...
متن کاملClostridium perfringens type D epsilon prototoxin. Some chemical, immunological and biological properties of a highly purified prototoxin.
WORTHINGTON, R. W., MOLDERS, MARIAS. G . & VAN RENSBURG, J . J. , 1973. Clostridium perfringens typeD epsilon prototoxin. Some chemical, immunological and biological properties of a highly purified preparation of proto toxin . Onderstepoort J. vet. Res. 40(4) 145152 (1973) Highly purified C/. perfringens typeD epsilon prototoxin was prepared by ammonium sulphate precipitation and DEAE cellulose...
متن کاملEpsilon-toxin plasmids of Clostridium perfringens type D are conjugative.
Isolates of Clostridium perfringens type D produce the potent epsilon-toxin (a CDC/U.S. Department of Agriculture overlap class B select agent) and are responsible for several economically significant enterotoxemias of domestic livestock. It is well established that the epsilon-toxin structural gene, etx, occurs on large plasmids. We show here that at least two of these plasmids are conjugative...
متن کاملFunctional analysis of neutralizing antibodies against Clostridium perfringens epsilon-toxin.
The Clostridium perfringens epsilon-toxin causes a severe, often fatal illness (enterotoxemia) characterized by cardiac, pulmonary, kidney, and brain edema. In this study, we examined the activities of two neutralizing monoclonal antibodies against the C. perfringens epsilon-toxin. Both antibodies inhibited epsilon-toxin cytotoxicity towards cultured MDCK cells and inhibited the ability of the ...
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ژورنال
عنوان ژورنال: Anaerobe
سال: 2012
ISSN: 1075-9964
DOI: 10.1016/j.anaerobe.2012.09.001